Purification, Cloning and Functional Characterization of Fructan: Fructan 1-Fructosyltransferase from Edible Burdock (Arctium lappa L.)
نویسندگان
چکیده
منابع مشابه
Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.)
BACKGROUND We have previously reported on the variation of total fructooligosaccharides (FOS), total inulooligosaccharides (IOS) and inulin in the roots of burdock stored at different temperatures. During storage at 0°C, an increase of FOS as a result of the hydrolysis of inulin was observed. Moreover, we suggested that an increase of IOS would likely be due to the synthesis of the IOS by fruct...
متن کاملA Novel Fructan Possessing DB Value from Roots of Arctium lappa L
A fructan named ALF was isolated from the roots of Arctium lappa L.. The structure of ALF was identified by analyses of methylation, GC-MS, and both 1 H and 13 C NMR spectroscopy. The results obtained indicated that ALF, comprised of D-fructose and D-glucose in the molar ratio of 14:1, was an inulin-type fructan, which was confirmed by the composition of 14 fructose residues linked by (2 1) gly...
متن کاملPurification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.).
Fructans, beta2-1 and/or beta2-6 linked polymers of fructose, are produced by fructosyltransferases (FTs) from sucrose. They are important storage carbohydrates in many plants. Fructan reserves, widely distributed in plants, are believed to be mobilized via fructan exohydrolases (FEHs). The purification, cloning, and functional characterization of a 6-FEH from wheat (Triticum aestivum L.) are r...
متن کاملCloning of sucrose:sucrose 1-fructosyltransferase from onion and synthesis of structurally defined fructan molecules from sucrose.
Sucrose (Suc):Suc 1-fructosyltransferase (1-SST) is the key enzyme in plant fructan biosynthesis, since it catalyzes de novo fructan synthesis from Suc. We have cloned 1-SST from onion (Allium cepa) by screening a cDNA library using acid invertase from tulip (Tulipa gesneriana) as a probe. Expression assays in tobacco (Nicotiana plumbaginifolia) protoplasts showed the formation of 1-kestose fro...
متن کاملCloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilization.
Fructans, which are beta-(2,1) and/or beta-(2,6) linked polymers of fructose, are important storage carbohydrates in many plants. They are mobilized via fructan exohydrolases (FEHs). The cloning, mapping, and functional analysis of the first 1-FEH (EC 3.2.1.153) from Lolium perenne L. var. Bravo is described here. By screening a perennial ryegrass cDNA library, a 1-FEH cDNA named Lp1-FEHa was c...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Applied Glycoscience
سال: 2009
ISSN: 1344-7882,1880-7291
DOI: 10.5458/jag.56.239